rotational thromboelastometry (whole blood coagulation analyzer; rotem-delta  (TEM International)

Journal: Journal of Clinical Medicine

Article Title: Perioperative Changes in Hemostatic Properties as Assessed by Multiplate, Siemens PFA-200, and ROTEM—A Comparative Study

doi: 10.3390/jcm14051640

Figure Lengend Snippet: Platelet function testing and rotational thromboelastometry data for the study cohort at both preoperative and postoperative (postoperative day 4—POD4) timepoints.

Article Snippet: , ROTEM Delta ® Rotational Thromboelastometry , , , , .

Techniques: FIBTEM Assay

Journal: Journal of Clinical Medicine

Article Title: Perioperative Changes in Hemostatic Properties as Assessed by Multiplate, Siemens PFA-200, and ROTEM—A Comparative Study

doi: 10.3390/jcm14051640

Figure Lengend Snippet: Spearman’s correlation test between preoperative point-of-care Siemens PFA-200, Multiplate, and ROTEM Delta assays and the presence of excessive bleeding.

Article Snippet: , ROTEM Delta ® Rotational Thromboelastometry , , , , .

Techniques: FIBTEM Assay

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Early posttraumatic hemostasis is not changed after GPIIb/IIIa inhibition via tirofiban. The in vivo inhibition of GPIIb/IIIa via tirofiban prior to burn injury does not change parameters assessed by thromboelastometry. Clotting time, indicating the onset of clot formation, alpha-angle, indicating the velocity of clot formation, and maximum clot firmness (MCF), indicating the clot stability, were measured ( a – c ). Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Inhibition, In Vivo, Coagulation

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Disruption of fibrinogen annihilates effective hemostasis. The disruption of fibrinogen annihilates hemostasis but the fibrinogenic pathway seems to be less affected than platelets, as in the thromboelastometry analysis, the fib-tem® activation, which inhibits platelet contribution to the clot firmness, is slightly sustained compared to ex-tem® activation. Activation of coagulation was conducted with ex-tem® and fib-tem®. The graphs representing the clotting process in the control group ( a ) and in the fibrinogen depletion group ( b ) were displayed. The alpha-angle and maximum clot firmness were compared between all groups and interventions ( c – f ). Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Activation Assay, Coagulation

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Early posttraumatic hemostasis is not differed following PAR4 disruption. The hemostasis assessed by thromboelastometry early post burn injury is not affected by PAR4 disruption. The clotting time (CT) ( a ), alpha-angle ( b ), and the maximum clot firmness (MCF) ( c ) were depicted. Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Coagulation

Journal: Veterinary Sciences

Article Title: Improved Cardiovascular Tolerance to Hemorrhage after Oral Resveratrol Pretreatment in Dogs

doi: 10.3390/vetsci8070129

Figure Lengend Snippet: Rotational Thromboelastometry (ROTEM ® ) viscoelastic and thrombin generation parameters of the anesthetized greyhound dogs before and after induced hemorrhage ( n = 9).

Article Snippet: Physical examination, renal ultrasonography, urinalysis, complete blood count, serum creatinine (SCr), blood urea nitrogen, serum albumin concentration, platelet closure time (PCT), and Rotational Thromboelastometry (ROTEM ® delta, Tem International GmbH, Munich, Germany) for all dogs were within reference intervals for adult Greyhounds [ ].

Techniques:

Journal: Veterinary Sciences

Article Title: Improved Cardiovascular Tolerance to Hemorrhage after Oral Resveratrol Pretreatment in Dogs

doi: 10.3390/vetsci8070129

Figure Lengend Snippet: Linear mixed models showing the associations between resveratrol treatment with maximum clot firmness on the Rotational Thromboelastometry (ROTEM ® ) of the anesthetized greyhound dogs before and after induced hemorrhage ( n = 12) after adjusting for volume of blood removed using unstructured covariance structure. MCF = maximum clot firmness.

Article Snippet: Physical examination, renal ultrasonography, urinalysis, complete blood count, serum creatinine (SCr), blood urea nitrogen, serum albumin concentration, platelet closure time (PCT), and Rotational Thromboelastometry (ROTEM ® delta, Tem International GmbH, Munich, Germany) for all dogs were within reference intervals for adult Greyhounds [ ].

Techniques:

Journal: Blood Advances

Article Title: Loss of GPVI and GPIbα contributes to trauma-induced platelet dysfunction in severely injured patients

doi: 10.1182/bloodadvances.2020001776

Figure Lengend Snippet: Platelet responses to collagen and CRP-XL in patients stratified by clinical outcome and presence of coagulopathy. (A) Area coverage after adhesion to type 1 collagen under flow conditions in survivors (n = 10) and nonsurvivors (n = 4). (B-C) Aggregation in response to type 1 (Horm) collagen (B) and CRP-XL (C) in survivors (n = 13) and nonsurvivors (n = 7). (D-E) Correlation between adhesion to collagen under flow and CA5 on ROTEM in the presence of tissue factor (EXTEM; D) and after subtraction of cytochalasin D assay to isolate platelet contribution to CA5 (EXTEM-FIBTEM; E). (F-G) Aggregation in response to collagen (F) and CRP-XL (G) in patients with (n = 13) and without (n = 7) TIC. Student t test (A) or 2-way ANOVA with Šidák’s multiple-comparisons test (B-C,F-G). *P < .05; **P < .01; ***P < .001.

Article Snippet: Thromboelastometry Rotational thromboelastometry (ROTEM delta instrument; Rotem, Leipzig, Germany) was performed with citrated whole blood after addition of tissue factor in the presence (FIBTEM) and absence (EXTEM) of the actin polymerization inhibitor cytochalasin D. The platelet contribution to each parameter was calculated by subtracting the FIBTEM value from the EXTEM value, as previously described.

Techniques: FIBTEM Assay

Journal: Blood Advances

Article Title: Loss of GPVI and GPIbα contributes to trauma-induced platelet dysfunction in severely injured patients

doi: 10.1182/bloodadvances.2020001776

Figure Lengend Snippet: sGPVI levels in trauma patients stratified by injury severity and coagulopathy. (A) sGPVI levels in patients with moderate (ISS ≤25; n = 13) and critical (ISS >25; n = 17) injuries. (B) sGPVI levels in patients with (n = 19) and without (n = 11) TIC. (C-D) Correlation between sGPVI levels and CA5 on ROTEM EXTEM (C) and plasma fibrinogen level (D). Bars indicate the mean with 95% CI. Student t test. *P < .05.

Article Snippet: Thromboelastometry Rotational thromboelastometry (ROTEM delta instrument; Rotem, Leipzig, Germany) was performed with citrated whole blood after addition of tissue factor in the presence (FIBTEM) and absence (EXTEM) of the actin polymerization inhibitor cytochalasin D. The platelet contribution to each parameter was calculated by subtracting the FIBTEM value from the EXTEM value, as previously described.

Techniques: Clinical Proteomics